| 质粒名称: | pLM-AMA18.0 dCas9-VPR |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 138945 |
| 存储实验室: | Arnold Driessen |
|
载体骨架基本信息 |
|
| 载体名称: | pDSM-JAK-109 |
| 载体出品公司: | DSM |
| 空载体大小: | 10170 bp |
| 完整质粒大小: | -- |
| 载体修饰: | pGpda:DsRed SKL已删除 |
| 载体类型: | CRISPR，合成生物学；AMA1自主复制真菌质粒 |
| 筛选标记: | 真菌选择：特比萘芬（ergA）/静脉霉素/博莱霉素（ble） |
| 载体抗性: | 氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers |
| 物种来源: | 合成的真菌表达，红色青霉 |
| 插入大小: | 21950 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | p40S（AN0465）巢状曲霉 |
| 标签/融合蛋白: | NLS |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLM-AMA18.0 dCas9-VPR was a gift from Arnold Driessen (Addgene plasmid # 138945 ; http://n2t.net/addgene:138945 ; RRID:Addgene_138945) |
| 参考文献部分: | CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM. Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. 10.1038/s41598-020-80864-3 PubMed 33441979 |
买家导航
pLM-AMA18.0 dCas9-VPR载体质粒基本信息
pLM-AMA18.0 dCas9-VPR质粒图谱载体图谱和pLM-AMA18.0 dCas9-VPR载体序列质粒序列多克隆位点信息
pLM-AMA18.0 dCas9-VPR质粒载体简介
pLM-AMA18.0 dCas9-VPR质粒序列载体序列
LOCUS pLM-AMA18.0 dCas9-VPR 21949 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta
(VPR); ribozymes based sgRNA transcription unit; Terbinafine and
Phleomycin selection markers.
ACCESSION .
VERSION .
KEYWORDS pLM-AMA18.0 dCas9-VPR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 21949)
AUTHORS Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen
AJM
TITLE CRISPR-based transcriptional activation tool for silent genes in
filamentous fungi.
JOURNAL Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3.
PUBMED 33441979
REFERENCE 2 (bases 1 to 21949)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..21949
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1415..5518
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=Cas9m4
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the mutations D10A, D839A,
H840A, and N863A (Mali et al., 2013)"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 5531..5551
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5576..5725
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
CDS 5750..5770
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6200..6556
/codon_start=1
/product="transcriptional activation domain of human RelA,
also known as p65 (O'Shea and Perkins, 2008)"
/label=RelA (p65) AD
/translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
EDFSSIADMDFSALL"
CDS 6575..7144
/codon_start=1
/product="transcriptional activation domain from the human
herpesvirus 4 (Epstein-Barr virus) replication and
transcription activator Rta/BRLF1 (Hardwick et al., 1992;
Chavez et al., 2015)"
/label=Rta AD
/translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
DTFLNDECLLHAMHISTGLSIFDTSLF"
intron 8316..8431
/gene="gpdA"
/label=gpdA intron
/note="intron from the Aspergillus nidulans
glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
1990)"
misc_RNA complement(10607..10682)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS complement(10776..11099)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
primer_bind 10781..10800
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 11009..11025
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 11026..11082
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(11095..11111)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 11119..11135
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11143..11173)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11188..11209
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
intron 11281..11396
/gene="gpdA"
/label=gpdA intron
/note="intron from the Aspergillus nidulans
glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
1990)"
primer_bind complement(11918..11937)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(11919..11937)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(11947..11963)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 12105..12560
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(12192..12211)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 12402..12423
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 12586..12690
/gene="bla"
/label=AmpR promoter
promoter 13010..13112
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 13113..13772
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(13179..13198)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
rep_origin 14161..14749
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 14650..14669
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 14903..14920
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 15037..15058
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 15073..15103
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 15111..15127
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 15135..15151
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 15170..15190
/label=T3
/note="T3 promoter, forward primer"
promoter 15172..15190
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 15982..16356
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
terminator 16441..16688
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(16457..16475)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
rep_origin 16698..21946
/label=AMA1
/note="replication origin from Aspergillus nidulans
(Aleksenko and Clutterbuck, 1996)"
ORIGIN
1 gaattcctgc agccccagat catcctgtct tcagtcttaa cgctgcaaga attcaagctt
61 ggaggagtta tagacggtcc ggcataggta aggagagatt agatatttcg acttcccgat
121 tccttagtct ccttagtctg gaaactgaga aaggcctgaa ctcgttcaat ctattagctc
181 taatcacgta gtctcaacta gaatcctgaa caggcgccgg aaccagtcca aatgcgtgga
241 gtacagcaag tccagtcaaa tcacgattag aatggccata tataagcaaa aaacccttgg
301 acaatacgtc cgatcgacac caaagcaatc tggtacatca cccttcttat atacagggta
361 gccggaattg tattgactct atacgaagcc gcaggtttac cagcacagag gtgcgatagc
421 tacacgaatg tagagtgaag gcattacaac cgattgctaa gtgtcataga ggctaagttt
481 cacggctcaa ggagtctgtg gaggatacct gatataagaa ttgagagtta tactccggag
541 gttatgttag tacctcaaaa atgtgaagta caacgaaact cgaacttcga atgacaatag
601 cttggctggc tgttagtcaa ctgaggtaca acgtcatctt gcaattagtt atgttctttg
661 ctagatagtg agctgctgta aacttgtagt tcttagaaac atgtctggag tctagagtcg
721 agacttgtga ttggtgtaaa ttagtggggg tccggagcgg aataacaatc gaggtcagcc
781 tcctgggtct gtcagtaagc taaccacgaa aggacaagcg tagacgaggt cacggggtac
841 tggcctagcc ggcttcggct agcgcccacg aaatagggca acgtcgaagc gagagacaca
901 cacggattcc acgatcgccg acgacaagca ctcaactcca cgacaaccca cgacaaccct
961 tgccgctgcg ttcttggtga agggctgcag gattagaggt gagtatttta ttttctggct
1021 attgtgatct ttttcccccc cggtttccgc tggttctatc ttgtcgagcg agtcggtgcg
1081 ccttcgatcg gtctgtcaaa tttccatccg tccactcgtt cacccggtat cgatacaaga
1141 gggaatggag cattacgaca ggggagcaga ggcagaactc tcgatgttgc tacttttttt
1201 gcttggaaca acttccttga aagattcttt tctgaatgcg gctgggaaac tgcgaattgg
1261 atatccagcg aaaggttacc gacagactca cggccatcga taccactcag cgggatagaa
1321 ttgaaggcat tcggacatta gatgatcagc ttcgcttgtt tcgactcgtg ctaatcaatt
1381 atccctggac agtcccccac atagacagca aacaatggac aagaagtatt ctatcggact
1441 ggccatcggg actaatagcg tcgggtgggc cgtgatcact gacgagtaca aggtgccctc
1501 taagaagttc aaggtgctcg ggaacaccga ccggcattcc atcaagaaaa atctgatcgg
1561 agctctcctc tttgattcag gggaaaccgc tgaagcaacc cgcctcaagc ggactgctag
1621 acggcggtac accaggagga agaaccggat ttgttacctt caagagatat tctccaacga
1681 aatggcaaag gtcgacgaca gcttcttcca taggctggaa gaatcattcc tcgtggaaga
1741 ggataagaag catgaacggc atcccatctt cggtaatatc gtcgacgagg tggcctatca
1801 cgagaaatac ccaaccatct accatcttcg caaaaagctg gtggactcaa ccgacaaggc
1861 agacctccgg cttatctacc tggccctggc ccacatgatc aagttcagag gccacttcct
1921 gatcgagggc gacctcaatc ctgacaatag cgatgtggat aaactgttca tccagctggt
1981 gcagacttac aaccagctct ttgaagagaa ccccatcaat gcaagcggag tcgatgccaa
2041 ggccattctg tcagcccggc tgtcaaagag ccgcagactt gagaatctta tcgctcagct
2101 gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt gctctttcac ttgggctgac
2161 tcccaatttc aagtctaatt tcgacctggc agaggatgcc aagctgcaac tgtccaagga
2221 cacctatgat gacgatctcg acaacctcct ggcccagatc ggtgaccaat acgccgacct
2281 tttccttgct gctaagaatc tttctgacgc catcctgctg tctgacattc tccgcgtgaa
2341 cactgaaatc accaaggccc ctctttcagc ttcaatgatt aagcggtatg atgagcacca
2401 ccaggacctg accctgctta aggcactcgt ccggcagcag cttccggaga agtacaagga
2461 aatcttcttt gaccagtcaa agaatggata cgccggctac atcgacggag gtgcctccca
2521 agaggaattt tataagttta tcaaacctat ccttgagaag atggacggca ccgaagagct
2581 cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag cgcactttcg acaatgggag
2641 cattccccac cagatccatc ttggggagct tcacgccatc cttcggcgcc aagaggactt
2701 ctaccccttt cttaaggaca acagggagaa gattgagaaa attctcactt tccgcatccc
2761 ctactacgtg ggacccctcg ccagaggaaa tagccggttt gcttggatga ccagaaagtc
2821 agaagaaact atcactccct ggaacttcga agaggtggtg gacaagggag ccagcgctca
2881 gtcattcatc gaacggatga ctaacttcga taagaacctc cccaatgaga aggtcctgcc
2941 gaaacattcc ctgctctacg agtactttac cgtgtacaac gagctgacca aggtgaaata
3001 tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt
3061 ggaccttctg ttcaagacca atagaaaggt gaccgtgaag cagctgaagg aggactattt
3121 caagaaaatt gaatgcttcg actctgtgga gattagcggg gtcgaagatc ggttcaacgc
3181 aagcctgggt acttaccatg atctgcttaa gatcatcaag gacaaggatt ttctggacaa
3241 tgaggagaac gaggacatcc ttgaggacat tgtcctgact ctcactctgt tcgaggaccg
3301 ggaaatgatc gaggagaggc ttaagaccta cgcccatctg ttcgacgata aagtgatgaa
3361 gcaacttaaa cggagaagat ataccggatg gggacgcctt agccgcaaac tcatcaacgg
3421 aatccgggac aaacagagcg gaaagaccat tcttgatttc cttaagagcg acggattcgc
3481 taatcgcaac ttcatgcaac ttatccatga tgattccctg acctttaagg aggacatcca
3541 gaaggcccaa gtgtctggac aaggtgactc actgcacgag catatcgcaa atctggctgg
3601 ttcacccgct attaagaagg gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa
3661 ggtgatgggt cgccataaac cagagaacat tgtcatcgag atggccaggg aaaaccagac
3721 tacccagaag ggacagaaga acagcaggga gcggatgaaa agaattgagg aagggattaa
3781 ggagctcggg tcacagatcc ttaaagagca cccggtggaa aacacccagc ttcagaatga
3841 gaagctctat ctgtactacc ttcaaaatgg acgcgatatg tatgtggacc aagagcttga
3901 tatcaacagg ctctcagact acgacgtggc tgccatcgtc cctcagagct tcctcaaaga
3961 cgactcaatt gacaataagg tgctgactcg ctcagacaag gcccggggaa agtcagataa
4021 cgtgccctca gaggaagtcg tgaaaaagat gaagaactat tggcgccagc ttctgaacgc
4081 aaagctgatc actcagcgga agttcgacaa tctcactaag gctgagaggg gcggactgag
4141 cgaactggac aaagcaggat tcattaaacg gcaacttgtg gagactcggc agattactaa
4201 acatgtcgcc caaatccttg actcacgcat gaataccaag tacgacgaaa acgacaaact
4261 tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg gtcagcgatt tcagaaagga
4321 ctttcaattc tacaaagtgc gggagatcaa taactatcat catgctcatg acgcatatct
4381 gaatgccgtg gtgggaaccg ccctgatcaa gaagtaccca aagctggaaa gcgagttcgt
4441 gtacggagac tacaaggtct acgacgtgcg caagatgatt gccaaatctg agcaggagat
4501 cggaaaggcc accgcaaagt acttcttcta cagcaacatc atgaatttct tcaagaccga
4561 aatcaccctt gcaaacggtg agatccggaa gaggccgctc atcgagacta atggggagac
4621 tggcgaaatc gtgtgggaca agggcagaga tttcgctacc gtgcgcaaag tgctttctat
4681 gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc ggaggctttt ctaaggaatc
4741 aatcctcccc aagcgcaact ccgacaagct cattgcaagg aagaaggatt gggaccctaa
4801 gaagtacggc ggattcgatt caccaactgt ggcttattct gtcctggtcg tggctaaggt
4861 ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa ctgctgggta tcaccattat
4921 ggagcgcagc tccttcgaga agaacccaat tgactttctc gaagccaaag gttacaagga
4981 agtcaagaag gaccttatca tcaagctccc aaagtatagc ctgttcgaac tggagaatgg
5041 gcggaagcgg atgctcgcct ccgctggcga acttcagaag ggtaatgagc tggctctccc
5101 ctccaagtac gtgaatttcc tctaccttgc aagccattac gagaagctga aggggagccc
5161 cgaggacaac gagcaaaagc aactgtttgt ggagcagcat aagcattatc tggacgagat
5221 cattgagcag atttccgagt tttctaaacg cgtcattctc gctgatgcca acctcgataa
5281 agtccttagc gcatacaata agcacagaga caaaccaatt cgggagcagg ctgagaatat
5341 catccacctg ttcaccctca ccaatcttgg tgcccctgcc gcattcaagt acttcgacac
5401 caccatcgac cggaaacgct atacctccac caaagaagtg ctggacgcca ccctcatcca
5461 ccagagcatc accggacttt acgaaactcg gattgacctc tcacagctcg gaggggatag
5521 cagggctgac cccaagaaga agaggaaggt ggaggccagc ggttccggac gggctgacgc
5581 attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga
5641 catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct
5701 tgatgatttc gacctggaca tgctgattaa ctctagaagt tccggatctc cgaaaaagaa
5761 acgcaaagtt ggtagccagt acctgcccga caccgacgac cggcaccgga tcgaggaaaa
5821 gcggaagcgg acctacgaga cattcaagag catcatgaag aagtccccct tcagcggccc
5881 caccgaccct agacctccac ctagaagaat cgccgtgccc agcagatcca gcgccagcgt
5941 gccaaaacct gccccccagc cttacccctt caccagcagc ctgagcacca tcaactacga
6001 cgagttccct accatggtgt tccccagcgg ccagatctct caggcctctg ctctggctcc
6061 agcccctcct caggtgctgc ctcaggctcc tgctcctgca ccagctccag ccatggtgtc
6121 tgcactggct caggcaccag cacccgtgcc tgtgctggct cctggacctc cacaggctgt
6181 ggctccacca gcccctaaac ctacacaggc cggcgagggc acactgtctg aagctctgct
6241 gcagctgcag ttcgacgacg aggatctggg agccctgctg ggaaacagca ccgatcctgc
6301 cgtgttcacc gacctggcca gcgtggacaa cagcgagttc cagcagctgc tgaaccaggg
6361 catccctgtg gcccctcaca ccaccgagcc catgctgatg gaataccccg aggccatcac
6421 ccggctcgtg acaggcgctc agaggcctcc tgatccagct cctgcccctc tgggagcacc
6481 aggcctgcct aatggactgc tgtctggcga cgaggacttc agctctatcg ccgacatgga
6541 cttctccgca ctgctgggta gcggatcggg atctcgggat tccagggaag ggatgttttt
6601 gccgaagcct gaggccggct ccgctattag tgacgtgttt gagggccgcg aggtgtgcca
6661 gccaaaacga atccggccat ttcatcctcc aggaagtcca tgggccaacc gcccactccc
6721 cgccagcctc gcaccaacac caaccggtcc agtacatgag ccagtcgggt cactgacccc
6781 ggcaccagtc cctcagccac tggatccagc gcccgcagtg actcccgagg ccagtcacct
6841 gttggaggat cccgatgaag agacgagcca ggctgtcaaa gcccttcggg agatggccga
6901 tactgtgatt ccccagaagg aagaggctgc aatctgtggc caaatggacc tttcccatcc
6961 gcccccaagg ggccatctgg atgagctgac aaccacactt gagtccatga ccgaggatct
7021 gaacctggac tcacccctga ccccggaatt gaacgagatt ctggatacct tcctgaacga
7081 cgagtgcctc ttgcatgcca tgcatatcag cacaggactg tccatcttcg acacatctct
7141 gttttgagct tacttcttta tcggttctct cttacgactt tttgaatgga acgtttcctt
7201 cttctcaggc gggcctatct ttgggccgaa gctcttttcc ttgtactgta ggacctggtt
7261 gataatgatt cccaaaaaga catccagcat gtcagttact tgcattcgtc agtctataca
7321 aaagcaatgg tttagagaaa ttttgaactt tatacatggt tttatttgtt gcttcacggc
7381 cgtaccttct ggaaatccac ggtaggagtg tcaatttgcg tttttgataa tccttccaag
7441 gttcttctcg aagtagttgt tctataattg cttcacagct accatggaac atcccaagca
7501 ccgctactag aattcgagct cggagtggat cccccgggct gcaggaattc gagctctgta
7561 cagtgaccgg tgactctttc tggcatgcgg agagacggac ggacgcagag agaagggctg
7621 agtaataagc gccactgcgc cagacagctc tggcggctct gaggtgcagt ggatgattat
7681 taatccggga ccggccgccc ctccgccccg aagtggaaag gctggtgtgc ccctcgttga
7741 ccaagaatct attgcatcat cggagaatat ggagcttcat cgaatcaccg gcagtaagcg
7801 aaggagaatg tgaagccagg ggtgtatagc cgtcggcgaa atagcatgcc attaacctag
7861 gtacagaagt ccaattgctt ccgatctggt aaaagattca cgagatagta ccttctccga
7921 agtaggtaga gcgagtaccc ggcgcgtaag ctccctaatt ggcccatccg gcatctgtag
7981 ggcgtccaaa tatcgtgcct ctcctgcttt gcccggtgta tgaaaccgga aaggccgctc
8041 aggagctggc cagcggcgca gaccgggaac acaagctggc agtcgaccca tccggtgctc
8101 tgcactcgac ctgctgaggt ccctcagtcc ctggtaggca gctttgcccc gtctgtccgc
8161 ccggtgtgtc ggcggggttg acaaggtcgt tgcgtcagtc caacatttgt tgccatattt
8221 tcctgctctc cccaccagct gctcttttct tttctctttc ttttcccatc ttcagtatat
8281 tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact
8341 ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc
8401 agcttgacta acagctaccc cgcttgagca gacatcacaa tgaccttgct caatggccac
8461 gcctccctct ccccggccgc tcaacgccgc atcgaacacc atgaagctga tattgtaatt
8521 gtgggtgccg gagtcctcgg ctgtgcactt gcagttgcac tgggaaatca agggcgcagt
8581 gtaatcctgc tggagaagtc gttggaagag cccaaccgta ttgttggtga actactccaa
8641 ccgggaggtg ttcaggcgct cgagcaacta gggctacggg attgtctgga agatatcgat
8701 ggcatcaatg tgaagggata ctgggttact tactttgggg agccggttct cctcgaatac
8761 cccaagtcca gcccaacctc gcctacaccg ttgggacgag ccttccacca tggccgattc
8821 gtgatgaaac tacgggcagc cgctctgtcg tgccccaatg tcacggtggt cgaaaccaag
8881 gttaccggcc tcatcacatc ctcatatact caggaagtcc ttggagtcga gtgtataacc
8941 aaggatgtca aggactgcta cttcggtcaa ctcaccgtgg ccgccgacgg ttataactcc
9001 gttttccgca aggaacacca ccagtataca cccaaacggc gctcaaagtt ccatggcttg
9061 gagctcatcg atgctaaact gcccgctccc aacactggtc atgtgcttct cagcgataat
9121 ccacccgtac ttatgtacca gatcggcacc catgaaaccc gaatccttat cgatatccct
9181 gataacctgc ctgcggcctc ggtcaagaac ggtggtgtca agggttacat gcgaaacaac
9241 atcctgccca agctcccaga gggtgtccag cagtcgttct ccgatgccct agagaagggc
9301 cagctacggt caatgccgaa ctcgttcctg cctgcctcgg ccaacaagac acccggcttg
9361 atgattctgg gcgatgcgct taacatgcgg caccctctca caggtggagg catgactgtg
9421 gcgctcaacg atgtttgtgt catccgcgaa ttgctcagcc cagagcgcgt gcccaacctc
9481 tccaatacag gccttgttct cgagcagctc gcagagttcc actggaaacg aaagaactca
9541 tcttcggtca tcaatatcct cgcacaggct ctctatgcgc tcttcgctgc agacagtacg
9601 cacgctcact cttcaggtta atttgcaata actaactgtt cattcaacag actattacct
9661 caaagctctt cagcgtggct gtttccgcta cttccagatt ggaccagttg gcggccctgt
9721 cgggttgtta gccggtctaa tcaagaaacc cctcgtcctt gtcagccact tcttctccgt
9781 tgccttcctc gccatctggg tccacatatg cgatacccct ctgtccaagc tattcctagc
9841 cccatactac gccgtcatga tattgtacac agcctctgtc gtgattttgc catacatctg
9901 gaccgagatc tggtactgag cttctaataa gtgtcagata gcaatttgca caagaaatca
9961 ataccagcaa ctgtaaataa gcgctgaagt gaccatgcca tgctacgaaa gagcagaaaa
10021 aaacctgccg tagaaccgaa gagatatgac acgcttccat ctctcaaagg aagaatccct
10081 tcagggttgc gtttccagtc tagacacgta taacggcaca agtgtctctc accaaatggg
10141 ttatatctca aatgtgatct aaggatggaa agcccagaat attggctggg ttgatggctg
10201 cttcgagtgc agtctcatgc tgccacaggt gactctggat ggccccatac cactcaaccc
10261 atggtacgag cggtacgctt tacgaattcc catggggagg agccaagagc ggattcctca
10321 gtctcgtacg tctcttgacg accgttgatc tgcttgatct cgtctcccga aaatgaaaat
10381 agactctgct aagctattct tctgcttcgc cggagcctga agggcgtact agggttgcga
10441 ggtccaatgc attaatgcat tgcagatgag ctgtatctgg aagaggtaaa cccgaaacgc
10501 gttttattct tgttgacatg gagctattaa atcagtccca ttcgccatgc cgaagcatgt
10561 tgcccagccg gcgccagcga ggaggctggg accatgccgg ccaaaagcac cgactcggtg
10621 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
10681 acggagacca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
10741 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
10801 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
10861 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
10921 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
10981 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
11041 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
11101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
11161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
11221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgggtctcg tcggtgatgt
11281 ctgctcaagc ggggtagctg ttagtcaagc tgcgatgaag tgggaaagct cgaactgaaa
11341 ggttcaaagg aataagggat gggaaggatg gagtatggat gtagcaaagt acttacttag
11401 gggaaataaa ggttcttgga tgggaagatg aatatactga agatgggaaa agaaagagaa
11461 aagaaaagag cagctggtgg ggagagcagg aaaatatggc aacaaatgtt ggactgacgc
11521 aacgaccttg tcaaccccgc cgacacaccg ggcggacaga cggggcaaag ctgcctacca
11581 gggactgagg gacctcagca ggtcgagtgt agagcaccgg atgggtcgac tgccagcttg
11641 tgttcccggt ctgcgccgct ggccagctcc tgagcggcct ttccggtttc atacaccggg
11701 caaagcagga gaggcacgat atttggacgc cctacagatg ccggatgggc caattaggga
11761 gcttacgccg ctcagagaat tcgcatgcgg agactttaga catacctctc gttgccgact
11821 taaggccgct ccccccctga aagagttgat attgaaggta gtaggtacgg tgcagggtaa
11881 cgcgtgatgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc
11941 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
12001 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
12061 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
12121 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
12181 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
12241 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
12301 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
12361 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
12421 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
12481 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
12541 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
12601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
12661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
12721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
12781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
12841 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
12901 cgaccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc
12961 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg
13021 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
13081 tgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
13141 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
13201 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
13261 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
13321 atccggaatt acgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
13381 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
13441 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
13501 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
13561 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
13621 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
13681 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
13741 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
13801 aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga
13861 aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta
13921 ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc
13981 ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcctttt
14041 tttctgatca aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
14101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
14161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
14221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
14281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
14341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
14401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
14461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
14521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
14581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
14641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
14701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
14761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
14821 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
14881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
14941 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
15001 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
15061 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg
15121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
15181 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgact acatgtatct
15241 gcatgttgca tcgggaaatc ccaccacagg gacagccaag cggccccgcg acttggcagt
15301 gggcaaacta cgcccgattc tggtgccaag aaccgagaag aatgagacag acccacgttg
15361 cactctaacc ggatgctatc gacttacggt ggctgaagat tcaacacgct gcaacgagag
15421 ccaaggtggt ccggacattt tctacgtgcc ggtttacctt ggaacatcgc cgtcgttgag
15481 tgcacgttgc ctactctctc gtggcttggc tgggcccacg agcccgattg actcgacggt
15541 gttacttggg tatctatggc cccgttttct ggcacggtaa tgataagtac ttactagtct
15601 tcgagcgggg gagtgttgct ctgcccgagc atcaacgatt ggcctgatcg caccgtctgc
15661 aaatgccacg gtgcggaccg actgaaatct cagaccacca aagaccctcc gacttcgaga
15721 tacggttact aattttacac tggctccagc ggccccatcc agtaagcatc tgggctgcaa
15781 gcgtataatg tctccaggtt gtctcagcat aaacaccccg cccccgctca ggcacacagg
15841 aagagagctc aggtcgtttc cattgcgtcc atactcttca ctcattgtca tctgcaggag
15901 aacttcccct gtccctttgc caagccctct cttcgtcgtt gtccacgcct tcaagttttc
15961 accattattt ttctagacac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
16021 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
16081 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
16141 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
16201 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
16261 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
16321 aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt
16381 ccgacgcggc ccgacgggtc cgaggcctcg gagatccgtc ccccttttcc tttgtcgata
16441 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg
16501 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt
16561 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt
16621 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt
16681 taatttgcaa gcttgatagc ttattttttg tatactgttt tgtgatagca cgaagttttt
16741 ccacggtatc ttgttaaaaa tatatatttg tggcgggctt acctacatca aattaataag
16801 agactaatta taaactaaac acacaagcaa gctactttag ggtaaaagtt tataaatgct
16861 tttgacgtat aaacgttgct tgtatttatt attacaatta aaggtggata gaaaacctag
16921 agactagtta gaaactaatc tcaggtttgc gttaaactaa atcagagccc gagaggttaa
16981 cagaacctag aaggggacta gatatccggg tagggaaaca aaaaaaaaaa acaagacagc
17041 cacatattag ggagactagt tagaagctag ttccaggact aggaaaataa aagacaatga
17101 taccacagtc tagttgacaa ctagatagat tctagattga ggccaaagtc tctgagatcc
17161 aggttagttg caactaatac tagttagtat ctagtctcct ataactctga agctagaata
17221 acttactact attatcctca ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt
17281 cagagactag ttattgacta gtcagtgact agcaataact aacaaggtat taacctacca
17341 tgtctgccat caccctgcac ttcctcgggc tcagcagcct tttcctcctc attttcatgc
17401 tcattttcct tgtttaagac tgtgactagt caaagactag tccagaacca caaaggagaa
17461 atgtcttacc actttcttca ttgcttgtct cttttgcatt atccatgtct gcaactagtt
17521 agagtctagt tagtgactag tccgacgagg acttgcttgt ctccggattg ttggaggaac
17581 tctccagggc ctcaagatcc acaacagagc cttctagaag actggtcaat aactagttgg
17641 tctttgtctg agtctgactt acgaggttgc atactcgctc cctttgcctc gtcaatcgat
17701 gagaaaaagc gccaaaactc gcaatatggc tttgaaccac acggtgctga gactagttag
17761 aatctagtcc caaactagct tggatagctt acctttgccc tttgcgttgc gacaggtctt
17821 gcagggtatg gttcctttct caccagctga tttagctgcc ttgctaccct cacggcggat
17881 ctgcataaag agtggctaga ggttataaat tagcactgat cctaggtacg gggctgaatg
17941 taacttgcct ttcctttctc atcgcgcggc aagacaggct tgctcaaatt cctaccagtc
18001 acaggggtat gcacggcgta cggaccactt gaactagtca cagattagtt agcaactagt
18061 ctgcattgaa tggctgtact tacgggccct cgccattgtc ctgatcattt ccagcttcac
18121 cctcgttgct gcaaagtagt tagtgactag tcaaggacta gttgaaatgg gagaagaaac
18181 tcacgaattc tcgacaccct tagtattgtg gtccttggac ttggtgctgc tatatattag
18241 ctaatacact agttagactc acagaaactt acgcagctcg cttgcgcttc ttggtaggag
18301 tcggggttgg gagaacagtg ccttcaaaca agccttcata ccatgctact tgactagtca
18361 gggactagtc accaagtaat ctagatagga cttgcctttg gcctccatca gttccttcat
18421 agtgggaggt ccattgtgca atgtaaactc catgccgtgg gagttcttgt ccttcaagtg
18481 cttgaccaat atgtttctgt tggcagaggg aacctgtcaa ctagttaata actagtcaga
18541 aactagtata gcagtagact cactgtacgc ttgaggcatc ccttcactcg gcagtagact
18601 tcatatggat ggatatcagg cacgccattg tcgtcctgtg gactagtcag taactaggct
18661 taaagctagt cgggtcggct tactatcttg aaatccggca gcgtaagctc cccgtcctta
18721 actgcctcga gatagtgaca gtactctggg gactttcgga gatcgttatc gcgaatgctc
18781 ggcatactaa tcgttgacta gtcttggact agtcccgagc aaaaaggatt ggaggaggag
18841 gaggaaggtg agagtgagac aaagagcgaa ataagagctt caaaggctat ctctaagcag
18901 tatgaaggtt aagtatctag ttcttgacta gatttaaaag agatttcgac tagttatgta
18961 cctggagttt ggatatagga atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa
19021 agtcggtcaa gaggtaactc taagtcggcc attccttttt gggaggcgct aaccataaac
19081 ggcatggtcg acttagagtt agctcaggga atttagggag ttatctgcga ccaccgagga
19141 acggcggaat gccaaagaat cccgatggag ctctagctgg cggttgacaa ccccaccttt
19201 tggcgtttct gcggcgttgc aggcgggact ggatacttcg tagaaccaga aaggcaaggc
19261 agaacgcgct cagcaagagt gttggaagtg atagcatgat gtgccttgtt aactaggtca
19321 aaatctgcag tatgcttgat gttatccaaa gtgtgagaga ggaaggtcca aacatacacg
19381 attgggagag ggcctaggta taagagtttt tgagtagaac gcatgtgagc ccagccatct
19441 cgaggagatt aaacacgggc cggcatttga tggctatgtt agtaccccaa tggaaagcct
19501 gagagtccag tggtcgcaga taactcccta aattccctga gctaactcta agtcgaccat
19561 gccgtttatg gttagcgcct cccaaaaagg aatggccgac ttagagttac ctcttgaccg
19621 actttttctt tcctccccct tacatttcgt taccacaaca cattcctata tccaaactcc
19681 aggtacataa ctagtcgaaa tctcttttaa atctagtcaa gaactagata cttaaccttc
19741 atactgctta gagatagcct ttgaagctct tatttcgctc tttgtctcac tctcaccttc
19801 ctcctcctcc tccaatcctt tttgctcggg actagtccaa gactagtcaa cgattagtat
19861 gccgagcatt cgcgataacg atctccgaaa gtccccagag tactgtcact atctcgaggc
19921 agttaaggac ggggagctta cgctgccgga tttcaagata gtaagccgac ccgactagct
19981 ttaagcctag ttactgacta gtccacagga cgacaatggc gtgcctgata tccatccata
20041 tgaagtctac tgccgagtga agggatgcct caagcgtaca gtgagtctac tgctatacta
20101 gtttctgact agttattaac tagttgacag gttccctctg ccaacagaaa catattggtc
20161 aagcacttga aggacaagaa ctcccacggc atggagttta cattgcacaa tggacctccc
20221 actatgaagg aactgatgga ggccaaaggc aagtcctatc tagattactt ggtgactagt
20281 ccctgactag tcaagtagca tggtatgaag gcttgtttga aggcactgtt ctcccaaccc
20341 cgactcctac caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta actagtgtat
20401 tagctaatat atagcagcac caagtccaag gaccacaata ctaagggtgt cgagaattcg
20461 tgagtttctt ctcccatttc aactagtcct tgactagtca ctaactactt tgcagcaacg
20521 agggtgaagc tggaaatgat caggacaatg gcgagggccc gtaagtacag ccattcaatg
20581 cagactagtt gctaactaat ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc
20641 tgtgactggt aggaatttga gcaagcctgt cttgccgcgc gatgagaaag gaaaggcaag
20701 ttacattcag ccccgtacct aggatcagtg ctaatttata acctctagcc actctttatg
20761 cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg aaccataccc
20821 tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt ttgggactag
20881 attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt
20941 ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag actcagacaa
21001 agaccaacta gttattgacc agtcttctag aaggctctgt tgtggatctt gaggccctgg
21061 agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca ctaactagac
21121 tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa agtggtaaga
21181 catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa acaaggaaaa
21241 tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag
21301 acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa taactagtct
21361 ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat aatagtagta
21421 agttattcta gcttcagagt tataggagac tagatactaa ctagtattag ttgcaactaa
21481 cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa ctagactgtg
21541 gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc tccctaatat
21601 gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc cttctaggtt
21661 ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt ttctaactag
21721 tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg tttatacgtc
21781 aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt ttataattag
21841 tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa caagataccg
21901 tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctatc
//
